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DEGRADATION OF INDUSTRIAL PINT EFFLUENT BY SOIL BACTERIA FROM ITS ENVIRONMENT

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DEGRADATION OF INDUSTRIAL PINT EFFLUENT BY SOIL BACTERIA FROM ITS ENVIRONMENT

 

Abstract

This work discusses degradation of industrial pint effluent by soil bacteria from its environment. Environmental pollution by oil-based paint effluents is of great concern nowadays due to widespread applications of oil-based paints that cause discharge and accidental spillages into the environment. The present study sought to explore the potential use of indigenous bacterial isolates for biodegradation of oil-based paints.

The oil-based-paint-biodegrading potential of three Bacil-lus species isolated from paint-polluted soil samples of different paint warehouses was investigated by the enu- meration, screening and taxonomic characterization of oil-based-paint-degrading  bacteria  using  soil  enrichment technique in mineral salt medium (MSM). The screened isolates were identified based on morphological, bio- chemical and 16S rRNA gene sequence homology meth-ods. The bioremediation potential of each bacterial isolate was determined for a period of 14 days at 37°C and 160 rpm in 250-ml shake flask containing 100 ml MSM plus oil paint (final conc. 300 ppm, w/v). The percent removal of oil-based paint was determined against a stan-dard curve of oil-based paint prepared by using UV–Vis spectrophotometer at 285 nm (k Max.).

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