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EXTRACTION, CHARACTERIZATION AND ANTI MICROBIAL SCREENING OF WHITE STAR APPLE (Crysophyllum Albidum) SEED OIL

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EXTRACTION, CHARACTERIZATION AND ANTI MICROBIAL SCREENING OF WHITE STAR APPLE (Crysophyllum Albidum) SEED OIL

 

ABSTRACT

Crysophylum albidum oil was extracted from its seed. The percentage yield was 2.56%. The characterization of the oil showed showed that the refractive index is 1.487, peroxide value is 45.4mg/kg, iodine value is 50.76g, saponification value is 105.188, free fatty acids 47.46 and acid value is 94.92. The Punched Ager Diffusion Method was used to assay for the antimicrobial and anti fungal properties of the oil in the test isolate. The antimicrobial and anti fungal activity showed some inhibitory effects against test organisms; Staphylococcus aureus, E. coli, B. subfilis, C. albican and A. flavons, but non for S. pyogens. The minimum inhibitory concentration of these test organisms are as follows; Staphylococcus aureus 0.16, E. coli 0.06, B. subfilis 0.14, C. albican 2.50 and A. flavons  0.40.

The pharmacological screening confirmed the medical value of this plant oil and it established a good support for the sample in herbal medicine and as a base for the development of new drugs and phytomedicine.

 

 

TABLE OF CONTENT

CONTENT

Title page

Table of content

Abstract

 

CHAPTER ONE

1.1 INTRODUCTION

LITERATURE REVIEW

1.2  TYPES OF OIL

1.2.1 Drying oil

1.2.2 Semi-Drying Oil

NON- DRYING OIL

 

1.3 COMPOSITION OF OIL

1.4 PIGMENT

VITAMINS  ANTIOXIDANTS

 

1.7  BOTANIC DESCRIPTION

1.8 FUNCTIONAL USES

 

CHAPTER TWO

MATERIALS AND METHODS

2.0  SAMPLE COLLECTION

2.1  OIL EXTRACTION

2.2 DETERMINATION OF SPECIFIC GRAVITY

2.3 DETERMINATION OF DENSITY

2.4  DETERMINATION OF FREE FATTY ACID

CONTENT

 

DETERMINATION OF SAPONIFICATION VALUE

DETERMINATION OF IODINE VALUE

DETERMINATION OF PEROXIDE VALUE

ANTI-MICROBIAL ACTIVITY SCREENING

REAGENTS AND MEDI

PREPARATION OF FUNGAL TEST ORGANISM

PREPARATION OF THE SENSITIVE TEST AGAR

PREPARATION OF THE NUTRIENT DEXTROSE AGAR

 

2.8.5 THE PUNCHED AGAR DIFFUSION METHOD AS  RECOMMENDED BY BRYANT (1972)

2.7.6 BACTERIAL INOCULATION AND INCUBATION

2.8.7 FUNGAL INOCULATION AND INCUBATION

READING INHIBITION ZONES OF THE OIL

 

CHAPTER THREE

TABLE 3.1 ORGANOLEPTIC CHARACTERISTICS OF  Crysophylum albidum SEED OIL

3.2 CHARACTERIZATION OF THE SAMPLE

 

TABLE 3.3 RESULT OF ANTI-BACTERIAL ACTIVITY OF C.  albidum OIL ON TWO GRAM POSITIVE BACTERI

 

TABLE 3.4 RESULT OF ANTI-BACTERIAL ACTIVITY OF C. albidum OIL ON TWO GRAM NEGATIVE BACTERI

 

TABLE 3.5 RESULT OF ANTI-FUNGAL ACTIVITY OF C. albidum  OIL ON TWO TEST FUNGI

3.6 RESULT OF MINIMUM INHIBITORY CONCENTRATION  (MIC) OF C. albidum OIL ON SIX TEST ORGANISM

 

CHAPTER FOUR

4.0  DISCUSSION

4.1 CONCLUSION

 

RECOMMENDATION

 

REFERENCES

APPENDIX

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