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PHYTOCHEMICAL AND ANTIMICROBIAL ANALYSIS OF MYSTETOE LEAVES

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PHYTOCHEMICAL AND ANTIMICROBIAL ANALYSIS OF MYSTETOE LEAVES

 

ABSTRACT

The sample of Mistletoe leaves was collected from an orange tree at Ururo in Umunze Anambra State. The leaves was extracted by using some solvent like ethanolic solvent, aqueous solvent n-hexane solvent and ethyl acetate solvent. The extracts was further analyzed which helps in determining the presence of phytochemical compounds in the Mistletoe plant leaf, such as alkaloids, flavonoid, phenol, tannins, steroid and saponins. These phytochemical compounds are found to be antimicrobial active agents. The efficiency of this plant in the treatment of various diseases necessitated the present work in order to identify the classes of natural products present in the leaf of the plant. These pharmacological values of this plant in herbal medicine and as base for the development of new drug.

 

TABLE OF CONTENT

Title page

Declaration

Certification

Dedication

Acknowledgment

Table of content

Abstract

 

CHAPTER ONE

1.0 INTRODUCTION

AIM AND OBJECTIVES

LITERATURE REVIEW OF COMMON

 

NAME MISTLE TOE

1.2  PLANT TAXONOMY

1.3  PHYTOCHEMICALS

1.3.1 TANINS

1.3.2 STEROIDS

1.3.3 PHENOLS

1.3.4 SAPONINS

1.3.5 FLAVONOIDS

1.3.6 ALKALOIDS

1.4 MEDICAL USES OF MISTLE TOE

CHAPTER TWO

2.0 MATERIALS AND METHODS

2.1  REAGENTS

2.2 CHEMICALS USED

2.3 VAPPARATUS USED

2.4 PHYTOCHEMICAL METHOD

2.5 PREPARATION OF THE SAMPLE

2.6 ETHANOLIC EXTRACT

2.7 N-HEXANE EXTRACT

2.8 AQEOUS EXTRACT

 

2.9 ETHYL ACETATE EXTRACT

2.10  CHEMICAL TESTS

2.10.1  TEST FOR ALKAKLOID

2.10.2 TEST FOR TANIN

2.10.3TEST FOR FLAVONOID

2.10.4 TEST FOR PHENOLS

2.10.5 TEST FOR STEROID

2.10.6 EMULSION TEST

2.10.7 TEST FOR SAPONIN

2.11 EXPERIMENTAL

2.11.1  PREPARATION OF SAMPLE

2.11.2  PREPARATION OF REAGENTS

2.11.3 EXTRACTION OF SAMPLE

2.11.4 AQUEOUS EXTRACT OF THE LEAVE

2.11.5  N-HEXANE EXTRACT OF THE LEAVES

2.11.6 ETHYL ACETATE EXTRACT OF THE LEAVE

2.12 CHEMICAL TESTS

2.12.1  ALKALOID

2.12.3  FLAVONOID

2.12.4  STEROID (SALKOWSKI TEST)

 

2.12.5 SAPONIN

2.12.6 EMIULSION TEST

2.13 EXTRACTION OF ALKALOIDS

2.14  METHOD OF SEPARATION

2.14  METHODS USED FOR THE TEST

AREAS FOLLOWS

CHAPTER THREE

3.0  RESULTS

3.1DISCUSSION ON PHYTOCHEMICAL RESULTSÂ

TABLE 1:   MINIMAL INHIBITION AND MINIMAL BATERICIDAL

CONCENTRATION OF THE FOUR EXTRACTS

TABLE 2:   MEAN DIAMETER OF ZONE OF INHIBITION (IN MM)

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CHAPTER FOUR

DISCUSSION

CONCLUSION

RECOMMENDATION

REFERENCES

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