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PRELIMINARY PHYTOCHEMICAL AND ANTIMICROBIAL SCREENING OF FLOWER AND STALK OF MANGIFERA INDICA (OPIORO MANGO)

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PRELIMINARY PHYTOCHEMICAL AND ANTIMICROBIAL SCREENING OF FLOWER AND STALK OF MANGIFERA INDICA (OPIORO MANGO)

 

ABSTRACT

Phytochemical and antimicrobial screening analysis of Mangifera indica indicated that the presence of the following moisture content 12.33% +0.0556%,  Alkaloid      7.35% +0.0291%, Tannins 18.22% +0.0070%, Saponic Glycoside 3.82% +0.0264%, Flanonoid  6.11% +0.0158%, Phenolic glycoside 0.010% + 0.0012%, Terpenoids 5.71% + 0.01%, Antrachionic 10.59% + 0.0158%,  pH 3.84% + 0.0529%. Cardiac Glycoside  7.90% + 0.0234%. Extracts of Mangifera indica were investigated for its antifungal and antibacterial activities. The aqueous extract did not inhibit any of the test fungi, the ethanolic extract produced zones of inhibition against Staphylococcus aureus, S. pyogens, Escherichia coli, Bacilus subfilis, while C. albican and a. flavus were not inhibited.

 

 

TABLE OF CONTENT

 

Title page

Declaration

Certification

Dedication

Acknowledgment

Table of content

Abstract

 

CHAPTER ONE

1.0INTRODUCTION

OBJECTIVE OF THIS RESEARCH

1.1 LITERATURE REVIEW

1.2    GENERAL USES AND IMPORTANCE OF

MANGIFERA INDIC

1.3 MORPHLOGY OF MANGIFERA INDICA; THE TREE AND ITS

FLOWERS

CHAPTER TWO

2.0 MATERIALS AND METHODS

2.1MATERIALS

2.2EQUIPMENTS AND APPARATUS

2.3  CHEMICAL REAGENTS

2.4 PHYTOCHEMICAL SCREENING (QUANTITATIVE ANALYSIS)

2.5 DETERMINATION OF SAPONIN

2.6 DETERMINATION OF ALKALOID

2.7  DETERMINATION OF TANINS

2.8  DETERMINATION OF MOISTURE CONTENT

2.9  DETERMINATION OF TOTAL ASH

2.10 CARDIAC GLYCOSIDE

2.11 TEST FOR ANTHRACENE GLYCOSIDES

2.12TERPENOIDS

2.13  pH AT 200C (10%)

CHAPTER THREE

TABLE 3.1 ORGANOLEPTIC  TEST  RESULT

TABLE 3.2 RESULT OF THE  PHYTOCHEMICAL  ANALYSIS

OF FLOWER AND STALK OF Mangifera Indica

TABLE 3.3RESULT OF ANTI-BACTERIAL ACTIVITY OF TWO

GRAM POSITIVE BACTERIA IN TWO CRUDE

EXTRACTS OF FLOWER AND STALK OF

Mangifera indica

TABLE 3.4 RESULT OF ANTI-BACTERIAL ACTIVITY OF

TWO GRAM NEGATIVE BACTERIA IN TWO

CRUDE EXTRACTS OF FLOWER AND STALK

OF Mangifera indica

TABLE 3.5 RESULT OF ANTI-FUNGAL ACTIVITY OF

TWO FUNGI IN TWO CRUDE EXTRACTS

OF FLOWER AND STALK

OF Mangifera indica

TABLE 3.6 RESULT OF MINIMUM INHIBITORY

CONCENTRATION

(MIC) OF CRUDE EXTRACT OF FLOWER

AND STALK

OF Mangifera indica

CHAPTER FOUR

DISCUSSION

CONCLUSION

RECOMMENDATION

REFERENCES

APPENDIX

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